Part:BBa_K4788008
FadR-FadBA-FadO-mRFP
This is an oleic acid inducer connected to the mRFP protein to characterize the effect of the oleic acid inducer.
Usage and Biology
The entire part is formed by adding the mRFP protein gene to the oleic acid inducer, which is used to characterize the oleic acid inducer. When the oleic acid inducer is activated, the mRFP protein will be expressed, and we can use fluorescence intensity to measure the expression level of the oleic acid inducer.
Characterization
To verify the effect of oleic acid inducer, we added mRFP gene after the oleic acid inducer gene to construct a reporter gene. We cultured engineering bacteria under two different environmental conditions, anaerobic and aerobic, and under different oleic acid induction conditions, and detected the expression effect of oleic acid inducers through normalized fluorescence signal intensity. As shown in Figure 1, the oleic acid inducer has a good response to gradient oleic acid, especially in the absence of oxygen, showing significant differences from the previous gradient at a 10% oleic acid gradient.
Under the comparison of anaerobic and aerobic conditions, as shown in Figure 2, there was a slight difference in the expression level of the oleic acid inducer gene, but it did not show significant difference.
Reference
Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 764
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1343
Illegal AgeI site found at 1455 - 1000COMPATIBLE WITH RFC[1000]
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